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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of the gene is observed in lots of tumors, which leads to chromosome instability, and thus could lead to tumorigenesis. This protein was discovered to affiliate with, and be associated with, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a essential part in advertising mobile-cycle development during the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, are actually described. Two supplemental splice variants were described but in-depth nucleotide sequence facts isn't nonetheless accessible.

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Blocking peptides are peptides that bind especially to your target antibody and block antibody binding. These peptide normally incorporates the epitope recognized with the antibody.

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This manual outlines further probable optimization of mobile and immunoassay parameters to make certain the best possible results are...

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This guidebook outlines even further doable optimization of cellular and immunoassay parameters to guarantee the very best effects are...

Antibodies sure to the blocking peptide no longer bind to your epitope around the goal protein. By comparing the staining through the blocked antibody compared to the antibody by itself, one can see which staining get more info is particular.

Concentrate on Data Cyclin E belongs towards the really conserved cyclin relatives, whose associates are characterized by a extraordinary periodicity in protein abundance from the cell cycle. Cyclins purpose as regulators of CDK kinases. Distinct cyclins show distinct expression and degradation designs which contribute to your temporal coordination of each and every mitotic celebration. Cyclin E types a posh with and functions being a regulatory subunit of CDK2, whose exercise is necessary for cell cycle G1/S transition. This protein accumulates within the G1-S stage boundary and it is degraded as cells progress by S stage.

The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by the two antibodies...

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